Healing of Generated Cracks in Cement Mortar Using MICP

Prakash B. Kulkarni, Pravin Dinkar Nemade, Manoj Pandurang Wagh


This research is carried out to investigate pre-existing repair cracks in cement mortar using the microbiologically induced calcium carbonate precipitation (MICP) technology. In the study, 20-cylinder mortar samples (45 mm in diameter and 40 mm in length) were split to have cracked width of various sizes. Out of twenty cracked samples, sixteen samples of average crack width ranging from 0.12 to 1.3 mm were repaired using the MICP method, while four cracked samples, with an average crack width ranging from 0.16 to 1.55 mm were soaked under distilled water. The water permeability and split tensile strength (STS) of these repaired mortars were tested. The amount of CaCO3 precipitated on the cracked mortar surfaces was evaluated. The results indicated that the MICP repair technique clearly reduced the water permeability of the cracked samples within the range of 73 to 84 %; while water-treated samples were too weak to undergo test. MICP-repaired samples had STS ranging from 29 to 380 kPa after 24 rounds of treatment. A relationship between the STS and percentage amount of CaCO3 precipitated was observed for samples with an average crack width between 0.29 and 1.1 mm, which indicated that STS increased with percentage increase in CaCO3 precipitated on the crack surfaces.


MICP; Split Tensile Strength; Cement Mortar; Permeability.


Van Tittelboom, Kim, Nele De Belie, Willem De Muynck, and Willy Verstraete. “Use of Bacteria to Repair Cracks in Concrete.” Cement and Concrete Research 40, no. 1 (January 2010): 157–166. doi:10.1016/j.cemconres.2009.08.025.

Wang, Jianyun, Kim Van Tittelboom, Nele De Belie, and Willy Verstraete. “Use of Silica Gel or Polyurethane Immobilized Bacteria for Self-Healing Concrete.” Construction and Building Materials 26, no. 1 (January 2012): 532–540. doi:10.1016/j.conbuildmat.2011.06.054.

Wang, J.Y., H. Soens, W. Verstraete, and N. De Belie. “Self-Healing Concrete by Use of Microencapsulated Bacterial Spores.” Cement and Concrete Research 56 (February 2014): 139–152. doi:10.1016/j.cemconres.2013.11.009.

Jonkers, Henk M., Arjan Thijssen, Gerard Muyzer, Oguzhan Copuroglu, and Erik Schlangen. “Application of Bacteria as Self-Healing Agent for the Development of Sustainable Concrete.” Ecological Engineering 36, no. 2 (February 2010): 230–235. doi:10.1016/j.ecoleng.2008.12.036.

Wiktor, Virginie, and Henk M. Jonkers. “Quantification of Crack-Healing in Novel Bacteria-Based Self-Healing Concrete.” Cement and Concrete Composites 33, no. 7 (August 2011): 763–770. doi:10.1016/j.cemconcomp.2011.03.012.

Khaliq, Wasim, and Muhammad Basit Ehsan. “Crack Healing in Concrete Using Various Bio Influenced Self-Healing Techniques.” Construction and Building Materials 102 (January 2016): 349–357. doi:10.1016/j.conbuildmat.2015.11.006.

Ramachandran S. K., V. Ramakrishnan, and S. S. Bang, “Remediation of Concrete Using Microorganisms.” ACI Materials Journal 98, no. 1 (2001): 3–9. doi:10.14359/10154.

Abo-El-Enein, S.A., A.H. Ali, Fatma N. Talkhan, and H.A. Abdel-Gawwad. “Utilization of Microbial Induced Calcite Precipitation for Sand Consolidation and Mortar Crack Remediation.” HBRC Journal 8, no. 3 (December 2012): 185–192. doi:10.1016/j.hbrcj.2013.02.001.

Achal, Varenyam, Abhijeet Mukerjee, and M. Sudhakara Reddy. “Biogenic Treatment Improves the Durability and Remediates the Cracks of Concrete Structures.” Construction and Building Materials 48 (November 2013): 1–5. doi:10.1016/j.conbuildmat.2013.06.061.

De Muynck, Willem, Dieter Debrouwer, Nele De Belie, and Willy Verstraete. “Bacterial Carbonate Precipitation Improves the Durability of Cementitious Materials.” Cement and Concrete Research 38, no. 7 (July 2008): 1005–1014. doi:10.1016/j.cemconres.2008.03.005.

Schlangen, H. E. J. G., H. M. Jonkers, S. Qian, and A. Garcia. "Recent advances on self healing of concrete." In FraMCos-7: Proceedings of the 7th International Conference on Fracture Mechanics of Concrete and Concrete Structures, Jeju Island, Korea, 23-28 May 2010. 2010.

Zahra Askari, Mehdi Asadi Aghbolaghi, Ali Hasantabar Amiri, Kaveh Ostad-Ali-Askari, and Saeid Eslamian, ”Crack Repair in Concrete Using Biological Methods”, American Research Journal of Civil and Structural Engineering 1, no. 1, (2017): 28-35.

Joshi, Sumit, Shweta Goyal, Abhijit Mukherjee, and M. Sudhakara Reddy. “Microbial Healing of Cracks in Concrete: a Review.” Journal of Industrial Microbiology & Biotechnology 44, no. 11 (September 12, 2017): 1511–1525. doi:10.1007/s10295-017-1978-0.

Pungrasmi, Wiboonluk, Jirapa Intarasoontron, Pitcha Jongvivatsakul, and Suched Likitlersuang. “Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete.” Scientific Reports 9, no. 1 (August 28, 2019). doi:10.1038/s41598-019-49002-6.

Sikder, Ankita, and Purnachandra Saha. "Effect of Bacteria on Performance of Concrete/Mortar: A Review." In International Conference on Advances in Civil Engineering (ICACE-2019), vol. 21, (2019): 12-17.

Chaurasia, Leena, Vishakha Bisht, L.P. Singh, and Sanjay Gupta. “A Novel Approach of Biomineralization for Improving Micro and Macro-Properties of Concrete.” Construction and Building Materials 195 (January 2019): 340–351. doi:10.1016/j.conbuildmat.2018.11.031.

Singh, L.P., Vishakha Bisht, M.S. Aswathy, Leena Chaurasia, and Sanjay Gupta. “Studies on Performance Enhancement of Recycled Aggregate by Incorporating Bio and Nano Materials.” Construction and Building Materials 181 (August 2018): 217–226. doi:10.1016/j.conbuildmat.2018.05.248.

Palin, D., V. Wiktor, and H.M. Jonkers. “Autogenous Healing of Marine Exposed Concrete: Characterization and Quantification through Visual Crack Closure.” Cement and Concrete Research 73 (July 2015): 17–24. doi:10.1016/j.cemconres.2015.02.021.

Qabany, A.AL, and K. Soga. “Effect of Chemical Treatment Used in MICP on Engineering Properties of Cemented Soils.” Géotechnique 63, no. 4 (March 2013): 331–339. doi:10.1680/geot.sip13.p.022.

Chen, How-Ji, Ching-Fang Peng, Chao-Wei Tang, and Yi-Tien Chen. “Self-Healing Concrete by Biological Substrate.” Materials 12, no. 24 (December 8, 2019): 4099. doi:10.3390/ma12244099.

Gavimath, C. C., B. M. Mali, V. R. Hooli, J. D. Mallpur, A. B. Patil, D. Gaddi, C. R. Ternikar, and B. E. Ravishankera. "Potential application of bacteria to improve the strength of cement concrete." International journal of advanced biotechnology and research 3, no. 1 (2012): 541-544.

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DOI: 10.28991/cej-2020-03091500


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